Part:BBa_K299806:Design

minC cell division inhibitor

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 652
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 652
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 652
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 652
  Illegal AgeI site found at 211
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

MinC under too strong RBS and strong promoter inhibits cell divisions in uncontrolled manner. Thus no transformant are generated using following construct: pT7-B0034-minC in BL21.

Source

Cloned by PCR amplification from E. coli BL21 genome